Exhibit 23.1

Consent of Independent Registered Public Accounting Firm

We consent to the incorporation by reference in the Registration Statement on Form S-1 filed pursuant to Rule 462(b) of the Securities Act of 1933 of the reference to our firm under the caption “Experts” and to the incorporation by reference of our report dated April 2, 2021 (except for paragraphs three through six of Note 17, as to which the date is June 21, 2021) with respect to the consolidated financial statements of Alpha Teknova, Inc. included in Amendment No. 1 to the Registration Statement (Form S-1 No. 333-256795) and related Prospectus of Alpha Teknova, Inc. for the registration of its common stock.

/s/ Ernst & Young LLP

San Jose, CA

June 24, 2021