Enumeral Biomedical Holdings, Inc. - FORM 8-K - EX-99.2 - EXHIBIT 99.2 - January 8, 2016

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8-K - 8-K - Enumeral Biomedical Holdings, Inc.s102453_8k.htm
EX-99.1 - EXHIBIT 99.1 - Enumeral Biomedical Holdings, Inc.s102453_ex99-1.htm

Exhibit 99.2

1sedforTCRsequencing.WedescribeamethodtomatchTCR sequenceswithTcellfunctionalprofilesthatincludescellsurface markers(e.g.CD3,CD4,CD8,TIGIT,PD-1)andsecretedcytokines (e.g. IL-6, TNF-a, IFN-?). Our method uses the proprietary EnumeralsinglecellplatformforbothTcellfunctionalprofiling andisolationofsingleTcellsofinterestforTCRsequencing.Here wedemonstratethatthismethodcanbeappliedtoTcellsfrom both blood and solid tumors, including lymphocytes from a metastaticmelanomapatient. Figure1.EnumeralPlatformbasedSingleTCellSelection. Tumorinfiltratedlymphocytes(TILs)wereanalyzedatsinglecelllevel on 85,000-well Enumeral array. Each single cell was p

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